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Understanding Microbiology Series
5 classes, April 27 through August 24, 2023
Course Description:
This series will focus on microbiological testing of environmental samples. The series will not train to the TNI Standard, but rather will cover basic and advanced microbiological principals and how to implement requirements of Volume 1 Module 5 of the 2016 TNI Standard. The courses in this series will cover an (1) introduction to microbiological testing as well as testing techniques, (2) quality control, equipment/instrumentation verification, and media reagent preparation, and (3) in-depth information on microbiological methods and technologies. Analysts and assessors seeking to expand their knowledge beyond the Standard can do so by learning in-depth information on microbiological testing.
PART 1: Introduction to Microbiological Testing
April 27, 2023, 11am-3pm EDT
This first course will provide participants with a general understanding of microbiological testing, techniques, method scopes and limitations, Quality Control, and technologies.
Topics addressed will include:
Microbiological Analytes
- Microbiological Sample Integrity
- Microbiological Lingo
- Microbiological Positive and Negative Controls
- Basic Microbiological Techniques Such as Serial Dilutions, Sterilization and Aseptic Technique
- Microbiological Test Methods Across Multiple Programs
- Microbiological Technologies and Associated Methods
Learning Objectives:
- Know microbiological testing is unique and important
- Know factors that can impact sample and data integrity
- Know terms and definitions used in microbiological testing
- Know basic microbiological quality control
- Know differences between microbiological testing techniques, methods and technologies in environmental samples
PART 2: Microbiological Testing Supplies, Equipment, and Instrumentation
May 25, 2023, 11am-12:30pm EDT
This course will provide participants with information on common microbiology equipment, instrumentation, and supplies. Verification of equipment, instrumentation and supplies will also be covered.
Topics addressed will include:
- Examples of equipment that will be discussed include but are not limited to: autoclaves, filtration apparatus, incubators/waterbaths, thermometers, dishwashers, and centrifuges
- Examples of instrumentation that will be discussed include but are not limited to microscopes, ELISA spectrophotometers, and thermocyclers
- Examples of supplies that will be discussed include but are not limited to pipets, petri dishes, and disposable funnels
Learning Objectives:
Have a general understanding of microbiological:
- Equipment
- Instrumentation
- Supplies
- The verification requirements for equipment, instrumentation, and supplies
PART 3: Microbiological Quality Control Testing
June 22, 2023, 11am-12:30pm EDT
This course will provide participants with information on microbiological quality control samples, trend analysis and control culture maintenance.
Learning Objectives:
Have a general understanding of microbiological batch Quality Control including:
- Positive/negative control samples
- Control samples such as method blanks, ongoing precision and recovery samples, and matrix spike/matrix spike duplicates
- Use of calibration curves
- Use of control charts
- Equivalent naming to chemistry control samples
- Reference culture creation and maintenance
PART 4: Microbiological Testing Media and Reagents
July 27, 2023, 11am-12:30pm EDT
This course will provide participants with information on creation and verification of microbiological testing media and reagents.
Learning Objectives:
Have a general understanding of microbiological media and reagents related to:
- How to make media and reagents
- Sterility testing
- Performance testing
- Storage Conditions
- Requirements related to Lab Made vs Pre-purchased Media
PART 5: Microbiological Testing Methods
August 24, 2023, 11am-2pm EDT
This course will provide participants with in depth information on microbiological testing methods.
Learning Objectives:
Have a general understanding of microbiological testing methods in relation to:
- The theory/science/history behind the methods
- What each analysis measures and reporting units
- Details related to each analysis including method-specific QC requirements
- Techniques for performing the methods
- Tips, tricks, and troubleshooting
- Pros and cons of various methods
- Applicable method confirmation testing
- Using/processing method data
Intended Audience:
- Analysts, Quality Managers, Technical Managers, Laboratory Managers
Cost:
$450 per person for TNI members
$500 per person for TNI non-members
$2000 per group of 5-10 students at same location for TNI members
$2250 per group of 5-10 students at same location for TNI non-members
No certificates are automatically provided with this training. A Certificate of Completion (CoC) ($30 per person) or Certificate of Attendance (CoA) ($20 per person) may be purchased during or after registration or webcast purchase. A passing score of at least 70% must be received on the exam in order to receive CEUs and a CoC. Scores of less than 70% will receive a Certificate of Attendance at no reduced fee. For a CoA you will not need to take the quiz and you will not receive CEUs. To receive your certificate, contact Paul Junio at [email protected] with date of purchase and date of training completion. See FAQs for additional information.
Format:
Webinar Start Date:
Thursday 27th April 2023, 12:00pm EDT Length:
11.5 hours CEUs: 1.2 
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Presented By:
TNI Microbiology Expert Committee About the Presenter:
Chair: Cody Danielson
The Microbiology Expert Committee develops and maintains consensus standards for microbiology that support TNI programs. |
The NELAC Institute
develop consensus standards for use by TNI's programs. This group has a support role in assisting other programs with activities such as guidance and standards interpretation.
fosters the generation of data of known and documented quality is through the National Environmental Laboratory Accreditation Program, or NELAP. The purpose of this program is to establish and implement a program for the accreditation of environmental laboratories.
is to establish and maintain a national PT program to support TNI’s National Environmental Laboratory Accreditation Program, and other activities.
Field Activities Program (NEFAP) Executive Committee is to establish and implement an accreditation program for field sampling and measurement organizations (FSMOs).
Audit Sample Program is to develop consensus standards for the manufacture and analysis of audit samples for source emission testing.
on a variety of topics of interest to the environmental measurement community. TNI also hosts two regularly scheduled meetings per year, typically in January and August. Both are a week long and involve open meetings of TNI committees as well as training courses and other special sessions.